Translational control by DHX36 binding to 5′UTR G-quadruplex is essential for muscle stem-cell regenerative functions

Activation-induced DHX36 is required for muscle formationTo dissect whether DHX36 functions in SCs and muscle regeneration, we first examined Dhx36 expression dynamics during SC myogenic progression. Quiescent SCs were isolated by fluorescence-activated cell sorting (FACS) from Pax7-nGFP mice25, either fixed in situ by 0.5% paraformaldehyde (PFA) prior to muscle digestion (SCT0) or from muscles digested [...]

Published August 18, 2021 at 07:58PM. Read full article at Stem Cells in the News Portal.