Downregulated lncRNA RCPCD promotes differentiation of embryonic stem cells into cardiac pacemaker-like cells by suppressing HCN4 promoter methylation

Cell cultureThe mouse ESCs were purchased from National Cell Resource Center (Shanghai, China) and cultured in Knockout Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% knockout Serum Replacement, 2 nM l-glutamine, 50 units/ml penicillin, 50 μg/ml streptomycin, 1% minimal essential medium nonessential amino acids, and β-mercaptoethanol, which are purchased from Thermo-Fisher and Sigma Company. The ESCs were cultured [...]

Published July 01, 2021 at 07:08PM. Read full article at Stem Cells in the News Portal.